Mushroom Grain To Grain Transfer

What Is A Grain To Grain Transfer

Grain to grain transfer is a simple and efficient process where colonized grain spawn is transferred to newly sterilized grain spawn for the purpose of expedited colonization of grain spawn.  This process can be repeated over and over again, which could result in huge amounts of colonized grain.

Advantages Of Grain To Grain Transfer

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Accelerated grain colonized

• Inoculating uncolonized grain with colonized grain results in much faster mycelium growth in comparison to inoculation with spore syringes
• Can be repeated several times, resulting in exponential growth of mushroom spawn
• More contact points with each individual grain

Cost-effective

• One jar of colonized spawn can be split into 10 jars of uncolonized spawn. This method allows for exponential growth of mushroom spawn, with the use of only one colonized spawn jar!

Easy & simple method

• Grain to grain transfer is a method used by beginners and advanced mycologists alike
• Not labour intensive, unlike making agar or liquid culture to use to inoculate
•  It does not require any special material or equipment, just a clean and sterilized work space. 

Downsides Of Grain To Grain Transfer

Contaminants 

• Without a flow hood, while doing grain to grain transfer, the grain may be exposed the the air during the transfer

Senescence

• Depending on the mushroom genetics, the number of grain to grain transfers could be limited. With the division of cells, there are arguments that the mycelium will weaken over many transfers. Which could possibly cause weaker colonization and fruiting.

Materials:

• Laminar flow hood or a SAB (still air box)
• Colonized mushroom spawn
• Sterilized grain 
• 70% isopropyl alcohol 

How To Perform Grain To Grain Transfer

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1. Sterilize work space and materials. Wipe down the jars/bags  of colonized mushroom spawn with 70% isopropyl alcohol. 
2. Break up colonized mushroom spawn for more evenly distribution
3. Using a laminar flow hood, or a still air box/glove box, carefully open up the shaken jar/bag of colonized spawn and evenly distribute the grain to the newly sterilized grain jars/bags. 
4. Seal the newly transferred jars/bags and give them a good shake to distribute the colonized grain for more contact points, which inturn leads to quicker colonization.
5. Store the newly transferred jars/bags in a dark and warm spot, full colonization should take 1-2 weeks.
6. Repeat after full colonization for exponential mushroom grain spawn growth.